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mouse VPS53 shRNA silencing AAV

Name 
AAV-m-VPS53-shRNA
Cat No 
shAAV-276074
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Vps53.

shAAV-276074
AAV-m-VPS53-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Vps53 (VPS53 GARP complex subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2010002A08Rik; 2310040I21Rik; 3100002B05Rik; Hccs1
Description (Vector)
VPS53 may be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.
Gene ID
68299
Gene Name (Vector)
VPS53 GARP complex subunit
Gene Symbol
Vps53
ORF Size (aa)
1968
ORF Size (bp)
1968 bp
RefSeq ID
NM_026664
RefSeq Synonyms
NM_026664, BC034371,
Species
mouse
UniGene ID
Mm.46397

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.