mouse UIMC1 shRNA silencing AAV

Name
AAV-m-UIMC1-shRNA

Cat No
shAAV-275614

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Uimc1.

shAAV-275614

AAV-m-UIMC1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Uimc1 (ubiquitin interaction motif containing 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

9430016E08Rik; D330018D10Rik; D630032M02Rik; RIP110; Rxrip110

Description (Vector)

Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly Act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.

Gene ID

20184

Gene Name (Vector)

ubiquitin interaction motif containing 1

Gene Symbol

Uimc1

ORF Size (aa)

2184

ORF Size (bp)

2184 bp

RefSeq ID

NM_011307

RefSeq Synonyms

NM_011307, NM_001293660, BC040808,

Species

mouse

UniGene ID

Mm.259301

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.