mouse TRPM8 shRNA silencing AAV

Name
AAV-m-TRPM8-shRNA

Cat No
shAAV-275100

Availability
Immediate

This is an AAV expressing shRNA for silencing of Mouse Trpm8.

shAAV-275100

AAV-m-TRPM8-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Trpm8 (transient receptor potential cation channel, subfamily M, member 8). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPP8

Description (Vector)

Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.

Gene ID

171382

Gene Name (Vector)

transient receptor potential cation channel, subfamily M, member 8

Gene Symbol

Trpm8

ORF Size (aa)

3315

ORF Size (bp)

3315 bp

RefSeq ID

NM_134252

RefSeq Synonyms

NM_134252, BC117934,

Species

mouse

UniGene ID

Mm.218753

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.