mouse TRIM25 shRNA silencing AAV

Name
AAV-m-TRIM25-shRNA

Cat No
shAAV-274977

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Trim25.

shAAV-274977

AAV-m-TRIM25-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Trim25 (tripartite motif-containing 25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AA960166; AL022677; EFP; Zfp147

Description (Vector)

Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs.

Gene ID

217069

Gene Name (Vector)

tripartite motif-containing 25

Gene Symbol

Trim25

ORF Size (aa)

1881

ORF Size (bp)

1881 bp

RefSeq ID

NM_009546

RefSeq Synonyms

NM_009546, BC034276, BC006908,

Species

mouse

UniGene ID

Mm.248445

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.