mouse TIMELESS shRNA silencing AAV

Name 
AAV-m-TIMELESS-shRNA
Cat No 
shAAV-274213
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Timeless.

shAAV-274213
AAV-m-TIMELESS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Timeless (timeless circadian clock 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C77407; Debt69; tim
Description (Vector)
Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.
Gene ID
21853
Gene Name (Vector)
timeless circadian clock 1
Gene Symbol
Timeless
ORF Size (aa)
3591
ORF Size (bp)
3591 bp
RefSeq ID
NM_011589
RefSeq Synonyms
NM_011589, NM_001164081, NM_001164080, NM_001136082, BC082770, BC058641,
Species
mouse
UniGene ID
Mm.6458

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.