mouse SPHK1 shRNA silencing AAV

Name 
AAV-m-SPHK1-shRNA
Cat No 
shAAV-272996
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Sphk1.

shAAV-272996
AAV-m-SPHK1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Sphk1 (sphingosine kinase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110006G24Rik; Sk1; Spk1
Description (Vector)
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Gene ID
20698
Gene Name (Vector)
sphingosine kinase 1
Gene Symbol
Sphk1
ORF Size (aa)
1149
ORF Size (bp)
1149 bp
RefSeq ID
NM_011451
RefSeq Synonyms
NM_025367, NM_011451, NM_001172475, NM_001172473, NM_001172472, BC037710,
Species
mouse
UniGene ID
Mm.20944

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.