mouse SETDB1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Setdb1.
shAAV-271764
AAV-m-SETDB1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Setdb1 (SET domain, bifurcated 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AU022152; ESET; KMT1E; mKIAA0067
Description (Vector)
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Gene ID
84505
Gene Name (Vector)
SET domain, bifurcated 1
Gene Symbol
Setdb1
ORF Size (aa)
2268
ORF Size (bp)
2268 bp
RefSeq ID
NM_018877
RefSeq Synonyms
NM_018877, NM_001163642, NM_001163641, BC079537,
Species
mouse
UniGene ID
Mm.477473
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.