mouse RNASEH2A shRNA silencing AAV

Name
AAV-m-RNASEH2A-shRNA

Cat No
shAAV-270649

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Rnaseh2a.

shAAV-270649

AAV-m-RNASEH2A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rnaseh2a (ribonuclease H2, large subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2400006P09Rik; RNASEHI; RNHIA; RNHL

Description (Vector)

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Gene ID

69724

Gene Name (Vector)

ribonuclease H2, large subunit

Gene Symbol

Rnaseh2a

ORF Size (aa)

765

ORF Size (bp)

765 bp

RefSeq ID

NM_027187

RefSeq Synonyms

NM_027187, BC038158, BC029252,

Species

mouse

UniGene ID

Mm.182470

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.