mouse RGMA shRNA silencing AAV

Name
AAV-m-RGMA-shRNA

Cat No
shAAV-270467

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Rgma.

shAAV-270467

AAV-m-RGMA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rgma (repulsive guidance molecule family member A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BC059072; C230063O06

Description (Vector)

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Gene ID

244058

Gene Name (Vector)

repulsive guidance molecule family member A

Gene Symbol

Rgma

ORF Size (aa)

1365

ORF Size (bp)

1365 bp

RefSeq ID

NM_177740

RefSeq Synonyms

NM_177740, BC059072, BC023870,

Species

mouse

UniGene ID

Mm.333943

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.