mouse RBM25 shRNA silencing AAV

Name
AAV-m-RBM25-shRNA

Cat No
shAAV-270240

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Rbm25.

shAAV-270240

AAV-m-RBM25-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Rbm25 (RNA binding motif protein 25). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; AU043498; RNPC7

Description (Vector)

RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA.

Gene ID

67039

Gene Name (Vector)

RNA binding motif protein 25

Gene Symbol

Rbm25

ORF Size (aa)

2526

ORF Size (bp)

2526 bp

RefSeq ID

NM_027349

RefSeq Synonyms

NM_027349, BC158104,

Species

mouse

UniGene ID

Mm.46005

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.