mouse POLR3F shRNA silencing AAV

Name 
AAV-m-POLR3F-shRNA
Cat No 
shAAV-269054
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Polr3f.

shAAV-269054
AAV-m-POLR3F-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Polr3f (polymerase (RNA) III (DNA directed) polypeptide F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2810411G20Rik; 3010019O03Rik; 3110032A07Rik; RPC39; RPC6
Description (Vector)
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Gene ID
70408
Gene Name (Vector)
polymerase (RNA) III (DNA directed) polypeptide F
Gene Symbol
Polr3f
ORF Size (aa)
951
ORF Size (bp)
951 bp
RefSeq ID
NM_029763
RefSeq Synonyms
NM_029763, BC009159,
Species
mouse
UniGene ID
Mm.389351

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.