mouse PARP1 shRNA silencing AAV

Name
AAV-m-PARP1-shRNA

Cat No
shAAV-268074

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Parp1.

shAAV-268074

AAV-m-PARP1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Parp1 (poly (ADP-ribose) polymerase family, member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

5830444G22Rik; Adprp; Adprt1; AI893648; ARTD1; C80510; PARP; parp-1; PPOL; sPARP-1

Description (Vector)

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Gene ID

11545

Gene Name (Vector)

poly (ADP-ribose) polymerase family, member 1

Gene Symbol

Parp1

ORF Size (aa)

3045

ORF Size (bp)

3045 bp

RefSeq ID

NM_007415

RefSeq Synonyms

NM_007415, BC012041,

Species

mouse

UniGene ID

Mm.277779

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.