mouse NGEF shRNA silencing AAV

Name 
AAV-m-NGEF-shRNA
Cat No 
shAAV-265744
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Ngef.

shAAV-265744
AAV-m-NGEF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ngef (neuronal guanine nucleotide exchange factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ephexin; Tims2
Description (Vector)
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
Gene ID
53972
Gene Name (Vector)
neuronal guanine nucleotide exchange factor
Gene Symbol
Ngef
ORF Size (aa)
2133
ORF Size (bp)
2133 bp
RefSeq ID
NM_019867
RefSeq Synonyms
NM_019867, NM_001111314, BC039279,
Species
mouse
UniGene ID
Mm.435439

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.