mouse NEDD4L shRNA silencing AAV

Name
AAV-m-NEDD4L-shRNA

Cat No
shAAV-265629

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Nedd4l.

shAAV-265629

AAV-m-NEDD4L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nedd4l (neural precursor cell expressed, developmentally down-regulated gene 4-like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

1300012C07Rik; Nedd4-2; Nedd4b

Description (Vector)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2.

Gene ID

83814

Gene Name (Vector)

neural precursor cell expressed, developmentally down-regulated gene 4-like

Gene Symbol

Nedd4l

ORF Size (aa)

2568

ORF Size (bp)

2568 bp

RefSeq ID

NM_031881

RefSeq Synonyms

NM_031881, NM_001114386, BC071210, BC039746,

Species

mouse

UniGene ID

Mm.98668

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.