mouse NDST3 shRNA silencing AAV

Name 
AAV-m-NDST3-shRNA
Cat No 
shAAV-265558
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Ndst3.

shAAV-265558
AAV-m-NDST3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ndst3 (N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4921531K01Rik; 4930511P15Rik; N-HSST 3; NDST-3
Description (Vector)
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.
Gene ID
83398
Gene Name (Vector)
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3
Gene Symbol
Ndst3
ORF Size (aa)
1929
ORF Size (bp)
1929 bp
RefSeq ID
NM_031186
RefSeq Synonyms
NM_031186, NM_001293682, BC112404, BC079622,
Species
mouse
UniGene ID
Mm.443908

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.