mouse NDE1 shRNA silencing AAV

Name 
AAV-m-NDE1-shRNA
Cat No 
shAAV-265536
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Nde1.

shAAV-265536
AAV-m-NDE1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nde1 (nudE neurodevelopment protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2810027M15Rik; AU042936; AW822251; mNudE; Nude
Description (Vector)
Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Gene ID
67203
Gene Name (Vector)
nudE neurodevelopment protein 1
Gene Symbol
Nde1
ORF Size (aa)
1035
ORF Size (bp)
1035 bp
RefSeq ID
NM_001114085
RefSeq Synonyms
NM_023317, NM_001285504, NM_001285503, NM_001114085, BC023267,
Species
mouse
UniGene ID
Mm.24105

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.