mouse LIG4 shRNA silencing AAV

Name
AAV-m-LIG4-shRNA

Cat No
shAAV-263448

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Lig4.

shAAV-263448

AAV-m-LIG4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Lig4 (ligase IV, DNA, ATP-dependent). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

5830471N16Rik; tiny

Description (Vector)

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Gene ID

319583

Gene Name (Vector)

ligase IV, DNA, ATP-dependent

Gene Symbol

Lig4

ORF Size (aa)

2733

ORF Size (bp)

2733 bp

RefSeq ID

NM_176953

RefSeq Synonyms

NM_176953, BC141417,

Species

mouse

UniGene ID

Mm.80584

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.