mouse LGTN shRNA silencing AAV

Name 
AAV-m-LGTN-shRNA
Cat No 
shAAV-263420
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Eif2d.

shAAV-263420
AAV-m-LGTN-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Eif2d (eukaryotic translation initiation factor 2D). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
D1Ertd5e; Lgtn
Description (Vector)
Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.
Gene ID
16865
Gene Name (Vector)
eukaryotic translation initiation factor 2D
Gene Symbol
Eif2d
ORF Size (aa)
1713
ORF Size (bp)
1713 bp
RefSeq ID
NM_010709
RefSeq Synonyms
NM_010709, NM_001136070, BC025036,
Species
mouse
UniGene ID
Mm.276294

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.