mouse LCAT shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Lcat.
shAAV-263294
AAV-m-LCAT-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Lcat (lecithin cholesterol acyltransferase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI046659; D8Wsu61e
Description (Vector)
Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms.
Gene ID
16816
Gene Name (Vector)
lecithin cholesterol acyltransferase
Gene Symbol
Lcat
ORF Size (aa)
1317
ORF Size (bp)
1317 bp
RefSeq ID
NM_008490
RefSeq Synonyms
NM_008490, BC028861,
Species
mouse
UniGene ID
Mm.1593
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.