mouse HCK shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Hck.
shAAV-261153
AAV-m-HCK-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Hck (hemopoietic cell kinase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI849071; Bmk
Description (Vector)
Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
Gene ID
15162
Gene Name (Vector)
hemopoietic cell kinase
Gene Symbol
Hck
ORF Size (aa)
1575
ORF Size (bp)
1575 bp
RefSeq ID
NM_010407
RefSeq Synonyms
NM_010407, NM_001172117, BC010478,
Species
mouse
UniGene ID
Mm.715
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.