mouse EIF2AK1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Eif2ak1.
shAAV-258180
AAV-m-EIF2AK1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
HCR; Hri
Description (Vector)
Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity).
Gene ID
15467
Gene Name (Vector)
eukaryotic translation initiation factor 2 alpha kinase 1
Gene Symbol
Eif2ak1
ORF Size (aa)
1860
ORF Size (bp)
1860 bp
RefSeq ID
NM_013557
RefSeq Synonyms
NM_013557, BC111035, BC028923,
Species
mouse
UniGene ID
Mm.431968
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.