mouse GAPDH shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Gapdh.
shAAV-257938
AAV-m-GAPDH-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Gapdh (glyceraldehyde-3-phosphate dehydrogenase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Gapd; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH
Description (eCommerce)
In the process of carbohydrate metabolism, GAPDH (glyceraldehydes-3-phosphate dehydrogenase) catalyzes the oxidative phosphorylation of glyceraldehydes-3-phosphate to 1,3-diphosphoglycerate in the presence of nicotinamide adenine dinucleotide (NAD) and inorganic phosphate. In addition to its role in the oxidation of glucose, GAPDH has been shown to be involved in other cellular processes such as vesicle transport, DNA repair, DNA replication, cytoskeletal modulation, and tRNA export.
Description (Vector)
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Gene ID
14433
Gene Name (eCommerce)
glyceraldehyde-3-phosphate dehydrogenase
Gene Name (Vector)
glyceraldehyde-3-phosphate dehydrogenase
Gene Symbol
Gapdh
NCBI Taxonomy ID (eCommerce)
10090.0
ORF Size (aa)
1002
ORF Size (bp)
1002 bp
Protein Name (eCommerce)
Glyceraldehyde-3-phosphate dehydrogenase
RefSeq ID
NM_008084
RefSeq Synonyms
NM_008084, NM_001289726, BC145812, BC145810, BC110311, BC096590, BC096440, BC095932, BC094037, BC093508, BC092294, BC092267, BC092264, BC092252, BC091768, BC085315, BC085275, BC085274, BC083149, BC083080, BC083079, BC083065, BC082592,
Research Areas
Alzheimer's Disease,Apoptosis,Breast Cancer,Cancer,Cytoskeleton,DNA Damage/Repair,HIF-1 Signaling,Metabolism/Metabolic Process,Neurobiology,Neurodegeneration,Neurodevelopment,Signal Transduction,Translational Control
Research Areas (Faceted)
cancer,cell_biology,developmental_biology,genetics,transcription_translation,metabolism,neurobiology,signal_transduction
Species
mouse
Target Sentence
In the process of carbohydrate metabolism, GAPDH (glyceraldehydes-3-phosphate dehydrogenase) catalyzes the oxidative phosphorylation of glyceraldehydes-3-phosphate to 1,3-diphosphoglycerate in the presence of nicotinamide adenine dinucleotide (NAD) and inorganic phosphate. In addition to its role in the oxidation of glucose, GAPDH has been shown to be involved in other cellular processes such as vesicle transport, DNA repair, DNA replication, cytoskeletal modulation, and tRNA export.
UniGene ID
Mm.458416
UniProt ID (eCommerce)
P16858
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.