mouse DPH1 shRNA silencing AAV

Name
AAV-m-DPH1-shRNA

Cat No
shAAV-257409

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Dph1.

shAAV-257409

AAV-m-DPH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Dph1 (diphthamide biosynthesis 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2310011M22Rik; 4930488F09Rik; AW551873; Dph2l1; Ovca1

Description (Vector)

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers (By similarity). Plays a role in embryonic growth, organogenesis and postnatal survival (By similarity).

Gene ID

116905

Gene Name (Vector)

diphthamide biosynthesis 1

Gene Symbol

Dph1

ORF Size (aa)

1314

ORF Size (bp)

1314 bp

RefSeq ID

NM_144491

RefSeq Synonyms

NM_144491, BC152729,

Species

mouse

UniGene ID

Mm.41496

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.