mouse DDX5 shRNA silencing AAV

Name
AAV-m-DDX5-shRNA

Cat No
shAAV-256956

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Ddx5.

shAAV-256956

AAV-m-DDX5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ddx5 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2600009A06Rik; G17P1; Hlr1; HUMP68; p68

Description (Vector)

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1.

Gene ID

13207

Gene Name (Vector)

DEAD (Asp-Glu-Ala-Asp) box polypeptide 5

Gene Symbol

Ddx5

ORF Size (aa)

1848

ORF Size (bp)

1848 bp

RefSeq ID

NM_007840

RefSeq Synonyms

NM_007840, BC129874, BC129873, BC108369, BC062916,

Species

mouse

UniGene ID

Mm.220038

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.