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mouse D2ERTD391E shRNA silencing AAV

Name 
AAV-m-D2ERTD391E-shRNA
Cat No 
shAAV-256678
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Atg13.

shAAV-256678
AAV-m-D2ERTD391E-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Atg13 (autophagy related 13). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110053A20Rik; D2Ertd391e; Harbi1
Description (Vector)
Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex.
Gene ID
51897
Gene Name (Vector)
autophagy related 13
Gene Symbol
Atg13
ORF Size (aa)
1440
ORF Size (bp)
1440 bp
RefSeq ID
NM_145528
RefSeq Synonyms
NM_145528, BC033419, BC023712, BC023673, BC016669,
Species
mouse
UniGene ID
Mm.28492

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.