mouse D10ERTD641E shRNA silencing AAV

Name 
AAV-m-D10ERTD641E-shRNA
Cat No 
shAAV-256613
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Anapc16.

shAAV-256613
AAV-m-D10ERTD641E-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Anapc16 (anaphase promoting complex subunit 16). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2310005G07Rik; APC16; D10Ertd641e
Description (Vector)
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Gene ID
52717
Gene Name (Vector)
anaphase promoting complex subunit 16
Gene Symbol
Anapc16
ORF Size (aa)
333
RefSeq ID
NM_025514
RefSeq Synonyms
NM_025514, NM_001347451, BC025117,
Species
mouse
UniGene ID
Mm.21793

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.