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mouse CHRNA9 shRNA silencing AAV

Name 
AAV-m-CHRNA9-shRNA
Cat No 
shAAV-255457
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Chrna9.

shAAV-255457
AAV-m-CHRNA9-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Chrna9 (cholinergic receptor, nicotinic, alpha polypeptide 9). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2410015I05Rik; Acra9; EG666827; Gm8311
Description (Vector)
Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.
Gene ID
231252
Gene Name (Vector)
cholinergic receptor, nicotinic, alpha polypeptide 9
Gene Symbol
Chrna9
ORF Size (aa)
1440
ORF Size (bp)
1440 bp
RefSeq ID
NM_001081104
RefSeq Synonyms
NM_001081104, BC153038, BC148446,
Species
mouse
UniGene ID
Mm.140015

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.