mouse CCDC99 shRNA silencing AAV

Name 
AAV-m-CCDC99-shRNA
Cat No 
shAAV-254812
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Spdl1.

shAAV-254812
AAV-m-CCDC99-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Spdl1 (spindle apparatus coiled-coil protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1700018I02Rik; 2600001J17Rik; 2810049B11Rik; AA409762; Ccdc99
Description (Vector)
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Gene ID
70385
Gene Name (Vector)
spindle apparatus coiled-coil protein 1
Gene Symbol
Spdl1
ORF Size (aa)
1827
ORF Size (bp)
1827 bp
RefSeq ID
NM_027411
RefSeq Synonyms
NM_027411, BC006674,
Species
mouse
UniGene ID
Mm.194368

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.