mouse CASP3 shRNA silencing AAV

Name 
AAV-m-CASP3-shRNA
Cat No 
shAAV-254558
Availability
Immediate
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Casp3.

shAAV-254558
AAV-m-CASP3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Casp3 (caspase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A830040C14Rik; AC-3; CASP-3; Caspase-3; CC3; CPP-32; CPP32; Lice; mldy; SCA-1; Yama
Description (Vector)
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Gene ID
12367
Gene Name (Vector)
caspase 3
Gene Symbol
Casp3
ORF Size (aa)
834
ORF Size (bp)
834 bp
RefSeq ID
NM_009810
RefSeq Synonyms
NM_009810, NM_001284409, BC038825,
Species
mouse
UniGene ID
Mm.34405

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.