mouse BAZ2A shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Baz2a.
shAAV-253640
AAV-m-BAZ2A-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Baz2a (bromodomain adjacent to zinc finger domain, 2A). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AA415431; C030005G16Rik; C78388; mKIAA0314; Tip5; Walp3
Description (Vector)
Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).
Gene ID
116848
Gene Name (Vector)
bromodomain adjacent to zinc finger domain, 2A
Gene Symbol
Baz2a
ORF Size (aa)
5661
ORF Size (bp)
5661 bp
RefSeq ID
NM_054078
RefSeq Synonyms
NM_054078, BC156872, BC148495,
Species
mouse
UniGene ID
Mm.252213
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.