mouse ATP5C1 shRNA silencing AAV

Name
AAV-m-ATP5C1-shRNA

Cat No
shAAV-253294

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Atp5c1.

shAAV-253294

AAV-m-ATP5C1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Atp5c1 (ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

1700094F02Rik

Description (Vector)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene ID

11949

Gene Name (Vector)

ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1

Gene Symbol

Atp5c1

ORF Size (aa)

897

ORF Size (bp)

897 bp

RefSeq ID

NM_020615

RefSeq Synonyms

NM_020615, NM_001112738, BC010700,

Species

mouse

UniGene ID

Mm.391281

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.