mouse ATP5A1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Atp5a1.
shAAV-253292
AAV-m-ATP5A1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Atp5a1 (ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI035633; AL022851; AL023067; Atpm; D18Ertd206e; Mom2
Description (Vector)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).
Gene ID
11946
Gene Name (Vector)
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
Gene Symbol
Atp5a1
ORF Size (aa)
1662
ORF Size (bp)
1662 bp
RefSeq ID
NM_007505
RefSeq Synonyms
NM_007505, BC014854,
Species
mouse
UniGene ID
Mm.474505
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.