mouse ATG5 shRNA silencing AAV

Name 
AAV-m-ATG5-shRNA
Cat No 
shAAV-253226
Availability
Immediate
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Atg5.

shAAV-253226
AAV-m-ATG5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Atg5 (autophagy related 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2010107M05Rik; 3110067M24Rik; Apg5l; Atg5l; AW319544; C88337; Paddy
Description (Vector)
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity)|Function: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Gene ID
11793
Gene Name (Vector)
autophagy related 5
Gene Symbol
Atg5
ORF Size (aa)
828
RefSeq ID
NM_053069
RefSeq Synonyms
NM_053069, NM_001314013, BC002166,
Species
mouse
UniGene ID
Mm.22264

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.