mouse ARHGAP10 shRNA silencing AAV

Name 
AAV-m-ARHGAP10-shRNA
Cat No 
shAAV-252928
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Arhgap10.

shAAV-252928
AAV-m-ARHGAP10-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Arhgap10 (Rho GTPase activating protein 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A930033B01Rik; PSGAP-m; PSGAP-s
Description (Vector)
GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).
Gene ID
78514
Gene Name (Vector)
Rho GTPase activating protein 10
Gene Symbol
Arhgap10
ORF Size (aa)
2358
ORF Size (bp)
2358 bp
RefSeq ID
NM_030113
RefSeq Synonyms
NM_030113, BC172679, BC156535,
Species
mouse
UniGene ID
Mm.165974

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.