mouse BRF1 shRNA silencing AAV

Name 
AAV-m-BRF1-shRNA
Cat No 
shAAV-251538
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Brf1.

shAAV-251538
AAV-m-BRF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Brf1 (BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2510002F24Rik; GTF3B; mTFIIIB90; TAF3C; TAFIII90; TFIIIB90
Description (Vector)
General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Gene ID
72308
Gene Name (Vector)
BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Gene Symbol
Brf1
ORF Size (aa)
2031
ORF Size (bp)
2031 bp
RefSeq ID
NM_028193
RefSeq Synonyms
NM_028193, BC037224,
Species
mouse
UniGene ID
Mm.196721

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.