mouse H2AFB1 shRNA silencing AAV

Name
AAV-m-H2AFB1-shRNA

Cat No
shAAV-250459

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse H2afb1.

shAAV-250459

AAV-m-H2AFB1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse H2afb1 (H2A histone family, member B1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

1700113O17Rik; H2A.Bbd; H2al2; TSEG-1; Tseg1

Description (Vector)

Atypical histone H2A which can replace conventional H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Nucleosomes containing this histone are less rigid and organize only 118 base pair of DNA instead of 147 in classical nucleosomes. They are associated with transcriptionally active chromatin and excluded form Barr bodies.

Gene ID

68231

Gene Name (Vector)

H2A histone family, member B1

Gene Symbol

H2afb1

ORF Size (aa)

336

ORF Size (bp)

336 bp

RefSeq ID

NM_026627

RefSeq Synonyms

NM_026627, BC061062,

Species

mouse

UniGene ID

Mm.23887

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.