human PEG3 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human PEG3.
shAAV-228262
AAV-h-PEG3-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human PEG3 (paternally expressed 3). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
PW1; ZKSCAN22; ZNF904; ZSCAN24
Description (Vector)
In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.
Gene ID
5178
Gene Name (Vector)
paternally expressed 3
Gene Symbol
PEG3
HGNC ID
HGNC:8826
ORF Size (aa)
4395
ORF Size (bp)
4395 bp
RefSeq ID
NM_006210
RefSeq Synonyms
NM_006210, NM_001146187, NM_001146186, NM_001146185, NM_001146184, BC150272, BC136268,
Species
human
UniGene ID
Hs.201776
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.