human ZIC4 shRNA silencing AAV

Name
AAV-h-ZIC4-shRNA

Cat No
shAAV-228257

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ZIC4.

shAAV-228257

AAV-h-ZIC4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ZIC4 (Zic family member 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

ZIC4 encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. ZIC4 is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene.

Gene ID

84107

Gene Name (Vector)

Zic family member 4

Gene Symbol

ZIC4

HGNC ID

HGNC:20393

ORF Size (aa)

1005

RefSeq ID

NM_032153

RefSeq Synonyms

NM_032153, NM_001243256, NM_001168379, NM_001168378, BC136339, BC126267,

Species

human

UniGene ID

Hs.415766

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.