human ZAP70 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human ZAP70.
shAAV-228030
AAV-h-ZAP70-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human ZAP70 (zeta chain of T cell receptor associated protein kinase 70). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; IMD48; SRK; STCD; STD; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; TZK; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Description (eCommerce)
ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.
Description (Vector)
ZAP70 encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Gene ID
7535
Gene Name (eCommerce)
zeta chain of T cell receptor associated protein kinase 70
Gene Name (Vector)
zeta chain of T cell receptor associated protein kinase 70
Gene Symbol
ZAP70
HGNC ID
HGNC:12858
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1482
ORF Size (bp)
1482 bp
Protein Name (eCommerce)
Tyrosine-protein kinase ZAP-70
RefSeq ID
NM_001079
RefSeq Synonyms
NM_207519, NM_001079, BC053878, BC039039,
Research Areas
Apoptosis,B-cell Receptor Signaling,EPO Signaling,IFN Signaling,Immunology,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Neurobiology,NF-kB Signaling,Phospho-specific,Prolactin Signaling,Signal Transduction,T-cell Receptor Signaling,VEGF Signaling
Research Areas (Faceted)
signal_transduction,cell_biology,immunology,signal_transduction,cell_biology,neurobiology,signal_transduction,immunology,cell_biology,signal_transduction,immunology
Species
human
Target Sentence
ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.
UniGene ID
Hs.234569
UniProt ID (eCommerce)
P43403
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.