human XBP1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human XBP1.
shAAV-227870
AAV-h-XBP1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human XBP1 (X-box binding protein 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
TREB-5; TREB5; XBP-1; XBP2
Description (Vector)
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. XBP1 product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Gene ID
7494
Gene Name (Vector)
X-box binding protein 1
Gene Symbol
XBP1
HGNC ID
HGNC:12801
ORF Size (aa)
786
RefSeq ID
NM_005080
RefSeq Synonyms
NM_005080, NM_001079539, BC012841, BC000938,
Species
human
UniGene ID
Hs.437638
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.