human VPS33B shRNA silencing AAV

Name
AAV-h-VPS33B-shRNA

Cat No
shAAV-227528

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human VPS33B.

shAAV-227528

AAV-h-VPS33B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VPS33B (VPS33B, late endosome and lysosome associated). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Gene ID

26276

Gene Name (Vector)

VPS33B, late endosome and lysosome associated

Gene Symbol

VPS33B

HGNC ID

HGNC:12712

ORF Size (aa)

1854

ORF Size (bp)

1854 bp

RefSeq ID

NM_018668

RefSeq Synonyms

NM_018668, NM_001289149, NM_001289148, BC016445,

Species

human

UniGene ID

Hs.718508

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.