human ALG11 shRNA silencing AAV

Name
AAV-h-ALG11-shRNA

Cat No
shAAV-227360

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ALG11.

shAAV-227360

AAV-h-ALG11-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ALG11 (ALG11, alpha-1,2-mannosyltransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CDG1P; GT8

Description (Vector)

ALG11 encodes a GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase which is localized to the cytosolic side of the endoplasmic reticulum (ER) and catalyzes the transfer of the fourth and fifth mannose residue from GDP-mannose (GDP-Man) to Man3GlcNAc2-PP-dolichol and Man4GlcNAc2-PP-dolichol resulting in the production of Man5GlcNAc2-PP-dolichol. Mutations in this gene are associated with congenital disorder of glycosylation type Ip (CDGIP). ALG11 overlaps but is distinct from the UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) gene. A pseudogene of the GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase has been identified on chromosome 19.

Gene ID

440138

Gene Name (Vector)

ALG11, alpha-1,2-mannosyltransferase

Gene Symbol

ALG11

HGNC ID

HGNC:32456

ORF Size (aa)

1476

ORF Size (bp)

1476 bp

RefSeq ID

NM_001004127

RefSeq Synonyms

NM_001004127, BC150316, BC143059, BC142998,

Species

human

UniGene ID

Hs.512963

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.