human USP20 shRNA silencing AAV

Name 
AAV-h-USP20-shRNA
Cat No 
shAAV-227294
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human USP20.

shAAV-227294
AAV-h-USP20-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human USP20 (ubiquitin specific peptidase 20). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
deubiquitinating enzyme 20; hVDU2; LSFR3A; pVHL-interacting deubiquitinating enzyme 2; ubiquitin carboxyl-terminal hydrolase 20; ubiquitin thioesterase 20; ubiquitin thiolesterase 20; ubiquitin-specific-processing protease 20; VDU2; VHL-interacting deubiquitinating enzyme 2
Description (eCommerce)
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 20 (USP20) is a UBP and is also called von-Hippel-Lindau protein (pVHL) interacting deubiquitinating enzyme 2 (VDU2). pVHL is an E3 ubiquitin-ligase that ubiquitinates hypoxia-inducible factor (HIF)-1alpha. USP20/VDU2 functions to specifically deubiquitinate HIF-1alpha to increase expression of HIF-1alpha regulated genes.
Gene ID
10868
Gene Name (eCommerce)
ubiquitin specific peptidase 20
Gene Name (Vector)
ubiquitin specific peptidase 20
Gene Symbol
USP20
HGNC ID
HGNC:12619
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2742
ORF Size (bp)
2742 bp
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase 20
RefSeq ID
NM_006676
RefSeq Synonyms
NM_006676, NM_001110303, NM_001008563, BC039593,
Research Areas
Cytoskeleton,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase 20 (USP20) is a UBP and is also called von-Hippel-Lindau protein (pVHL) interacting deubiquitinating enzyme 2 (VDU2). pVHL is an E3 ubiquitin-ligase that ubiquitinates hypoxia-inducible factor (HIF)-1alpha. USP20/VDU2 functions to specifically deubiquitinate HIF-1alpha to increase expression of HIF-1alpha regulated genes.
UniGene ID
Hs.5452
UniProt ID (eCommerce)
Q9Y2K6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.