human UBE4B shRNA silencing AAV

Name 
AAV-h-UBE4B-shRNA
Cat No 
shAAV-227054
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human UBE4B.

shAAV-227054
AAV-h-UBE4B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UBE4B (ubiquitination factor E4B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
E4; HDNB1; homologous to yeast UFD2; Homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; RING-type E3 ubiquitin transferase E4 B; ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitin-fusion degradation protein 2; UBOX3; UFD2; UFD2A; UFD2A-III/UBE4B-III splice isoform
Description (eCommerce)
Ubiquitin conjugation factor E4 B (UBE4B) is an E4 enzyme of the multi-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. The E4 enzyme is an additional conjugation factor which is involved in polyubiquitin chain assembly. UBE4B has been identified as a candidate neuroblastoma tumor suppressor gene.
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene ID
10277
Gene Name (eCommerce)
ubiquitination factor E4B
Gene Name (Vector)
ubiquitination factor E4B
Gene Symbol
UBE4B
HGNC ID
HGNC:12500
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3522
ORF Size (bp)
3522 bp
Protein Name (eCommerce)
Ubiquitin conjugation factor E4 B
RefSeq ID
NM_006048
RefSeq Synonyms
NM_006048, NM_001105562, BC093696,
Research Areas
Apoptosis,Cancer,Cardiac Development,Cardiology,Cardiovascular,Neurobiology,Neurodevelopment,Tumor Suppressors/Oncoproteins,Ubiquitination
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,neurobiology
Species
human
Target Sentence
Ubiquitin conjugation factor E4 B (UBE4B) is an E4 enzyme of the multi-step ubiquitination pathway. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. The E4 enzyme is an additional conjugation factor which is involved in polyubiquitin chain assembly. UBE4B has been identified as a candidate neuroblastoma tumor suppressor gene.
UniGene ID
Hs.632370
UniProt ID (eCommerce)
O95155

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.