human UBA6 shRNA silencing AAV

Name 
AAV-h-UBA6-shRNA
Cat No 
shAAV-226966
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human UBA6.

shAAV-226966
AAV-h-UBA6-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UBA6 (ubiquitin like modifier activating enzyme 6). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
E1-L2; monocyte protein 4; MOP-4; UBA6, ubiquitin-activating enzyme E1; UBE1L2; ubiquitin-activating enzyme 6; ubiquitin-activating enzyme E1-like 2; ubiquitin-activating enzyme E1-like protein 2; ubiquitin-like modifier-activating enzyme 6
Description (eCommerce)
Ubiquitin-like modifier-activating enzyme 6 (UBA6) activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. UBA6 is specific for ubiquitin and does not activate ubiquitin-like peptides. UBA6 differs from UBE1 in its specificity for substrate E2 charging. UBA6 does not charge cell cycle E2s, such as CDC34. UBE6 is essential for embryonic development and required for UBD/FAT10 conjugation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/A0AVT1].
Description (Vector)
Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3). UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins (Jin et al., 2007 (PubMed 17597759)).(supplied by OMIM, Mar 2008)
Gene ID
55236
Gene Name (eCommerce)
ubiquitin like modifier activating enzyme 6
Gene Name (Vector)
ubiquitin like modifier activating enzyme 6
Gene Symbol
UBA6
HGNC ID
HGNC:25581
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3159
ORF Size (bp)
3159 bp
Protein Name (eCommerce)
Ubiquitin-like modifier-activating enzyme 6
RefSeq ID
NM_018227
RefSeq Synonyms
NM_018227, BC126486, BC126484, BC031637,
Research Areas
Antigen Processing and Presentation,Immunology,Ubiquitination
Research Areas (Faceted)
cell_biology,immunology
Species
human
Target Sentence
Ubiquitin-like modifier-activating enzyme 6 (UBA6) activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. UBA6 is specific for ubiquitin and does not activate ubiquitin-like peptides. UBA6 differs from UBE1 in its specificity for substrate E2 charging. UBA6 does not charge cell cycle E2s, such as CDC34. UBE6 is essential for embryonic development and required for UBD/FAT10 conjugation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/A0AVT1].
UniGene ID
Hs.212774
UniProt ID (eCommerce)
A0AVT1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.