human TSG101 shRNA silencing AAV

Name 
AAV-h-TSG101-shRNA
Cat No 
shAAV-226598
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TSG101.

shAAV-226598
AAV-h-TSG101-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TSG101 (tumor susceptibility 101). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ESCRT-I complex subunit TSG101; TSG10; tumor susceptibility gene 10; tumor susceptibility gene 101; tumor susceptibility gene 101 protein; tumor susceptibility protein; VPS23
Description (eCommerce)
Tumor susceptibility gene 101 protein (TSG101) is a component of the ESCRT-I complex, a regulator of vesicular trafficking process. TSG101 mediates the association between the ESCRT-0 and ESCRT-I complex. TSG101 binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). TSG101, along with CEP55, is required for completion of cytokinesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99816].
Description (Vector)
TSG101 belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Gene ID
7251
Gene Name (eCommerce)
tumor susceptibility 101
Gene Name (Vector)
tumor susceptibility 101
Gene Symbol
TSG101
HGNC ID
HGNC:15971
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1173
Protein Name (eCommerce)
Tumor susceptibility gene 101 protein
RefSeq ID
NM_006292
RefSeq Synonyms
NM_006292, BC002487,
Research Areas
Cell Cycle,Cell Division,Host-Virus Interactions,Immunology,Lysosomal Transport,Nuclear Receptor Coactivators,T-cell Receptor Signaling,Transcription Factor/Regulator,Ubiquitination
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,infectious_disease,immunology,cell_biology,signal_transduction,immunology,genetics,transcription_translation
Species
human
Target Sentence
Tumor susceptibility gene 101 protein (TSG101) is a component of the ESCRT-I complex, a regulator of vesicular trafficking process. TSG101 mediates the association between the ESCRT-0 and ESCRT-I complex. TSG101 binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). TSG101, along with CEP55, is required for completion of cytokinesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99816].
UniGene ID
Hs.523512
UniProt ID (eCommerce)
Q99816

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.