human TRIM5 shRNA silencing AAV

Name 
AAV-h-TRIM5-shRNA
Cat No 
shAAV-226451
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TRIM5.

shAAV-226451
AAV-h-TRIM5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TRIM5 (tripartite motif containing 5). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
RNF88; TRIM5alpha
Description (Vector)
TRIM5 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Gene ID
85363
Gene Name (Vector)
tripartite motif containing 5
Gene Symbol
TRIM5
HGNC ID
HGNC:16276
ORF Size (aa)
1044
RefSeq ID
NM_033034
RefSeq Synonyms
NM_033093, NM_033092, NM_033034, BC021258,
Species
human
UniGene ID
Hs.370515

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.