human TRIM5 shRNA silencing AAV

Name
AAV-h-TRIM5-shRNA

Cat No
shAAV-226451

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human TRIM5.

shAAV-226451

AAV-h-TRIM5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TRIM5 (tripartite motif containing 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

RNF88; TRIM5alpha

Description (Vector)

TRIM5 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Gene ID

85363

Gene Name (Vector)

tripartite motif containing 5

Gene Symbol

TRIM5

HGNC ID

HGNC:16276

ORF Size (aa)

1044

RefSeq ID

NM_033034

RefSeq Synonyms

NM_033093, NM_033092, NM_033034, BC021258,

Species

human

UniGene ID

Hs.370515

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.