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human TMEM165 shRNA silencing AAV

Name 
AAV-h-TMEM165-shRNA
Cat No 
shAAV-225780
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TMEM165.

shAAV-225780
AAV-h-TMEM165-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TMEM165 (transmembrane protein 165). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CDG2K; FT27; GDT1; TMPT27; TPARL
Description (Vector)
TMEM165 encodes a predicted transmembrane protein with a perinuclear Golgi-like distribution in fibroblasts. Mutations in this gene are associated with the autosomal recessive disorder congenital disorder of glycosylation, type IIk. Knockdown of this gene's expression causes decreased sialylation in HEK cells and suggests this gene plays a role in terminal Golgi glycosylation. Alternative splicing results in multiple transcript variants.
Gene ID
55858
Gene Name (Vector)
transmembrane protein 165
Gene Symbol
TMEM165
HGNC ID
HGNC:30760
ORF Size (aa)
975
RefSeq ID
NM_018475
RefSeq Synonyms
NM_018475, BC107582, BC104980, BC104978, BC003545,
Species
human
UniGene ID
Hs.479766

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.