human TIMELESS shRNA silencing AAV

Name 
AAV-h-TIMELESS-shRNA
Cat No 
shAAV-225506
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TIMELESS.

shAAV-225506
AAV-h-TIMELESS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TIMELESS (timeless circadian clock). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hTIM; protein timeless homolog; TIM; TIM1; timeless circadian clock 1; timeless homolog; Tof1 homolog
Description (eCommerce)
Timeless was first identified in Drosophila as a transcriptional regulator involved in the fly circadian rhythm. In mice, humans and yeast, the homologs of drosophila timeless have been observed to play a role in the DNA damage checkpoint response. Human timeless has been shown to interact with the circadian clock protein cryptochrome 2 as well as the DNA checkpoint proteins Chk1 and the ATR-ATRIP complex suggesting a link between circadian rhythm and the cell cycle.
Gene ID
8914
Gene Name (eCommerce)
timeless circadian regulator
Gene Name (Vector)
timeless circadian clock
Gene Symbol
TIMELESS
HGNC ID
HGNC:11813
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3627
ORF Size (bp)
3627 bp
Protein Name (eCommerce)
Protein timeless homolog
RefSeq ID
NM_003920
RefSeq Synonyms
NM_003920, NM_001330295, BC050557,
Research Areas
Cell Cycle,Cell Division,Chromatin Remodeling,DNA Damage/Repair,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Timeless was first identified in Drosophila as a transcriptional regulator involved in the fly circadian rhythm. In mice, humans and yeast, the homologs of drosophila timeless have been observed to play a role in the DNA damage checkpoint response. Human timeless has been shown to interact with the circadian clock protein cryptochrome 2 as well as the DNA checkpoint proteins Chk1 and the ATR-ATRIP complex suggesting a link between circadian rhythm and the cell cycle.
UniGene ID
Hs.118631
UniProt ID (eCommerce)
Q9UNS1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.