human TCEB2 shRNA silencing AAV

Name 
AAV-h-TCEB2-shRNA
Cat No 
shAAV-225193
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ELOB.

shAAV-225193
AAV-h-TCEB2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ELOB (elongin B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
EloB; elongin 18 kDa subunit; elongin, 18-kD subunit; elongin-B; epididymis secretory sperm binding protein; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII; SIII p18; TCEB2; transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); transcription elongation factor B polypeptide 2; transcription elongation factor B subunit 2
Description (eCommerce)
TCEB2, also known as Elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation [taken from NCBI Entrez Gene (Gene ID: 6923)].
Description (Vector)
TCEB2 encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.
Gene ID
6923
Gene Name (eCommerce)
elongin B
Gene Name (Vector)
elongin B
Gene Symbol
ELOB
HGNC ID
HGNC:11619
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
357
Protein Name (eCommerce)
Transcription elongation factor B polypeptide 2
RefSeq ID
NM_007108
RefSeq Synonyms
NM_207013, NM_007108, BC065000, BC013306,
Research Areas
Antigen Processing and Presentation,Cancer,Cell Biology,Gene Expression,HIF-1 Signaling,Hypoxia,Immunology,Kidney Cancer,Signal Transduction,Transcription Factor/Regulator,Ubiquitination
Research Areas (Faceted)
cancer,cell_biology,immunology,signal_transduction,transcription_translation
Species
human
Target Sentence
TCEB2, also known as Elongin B, is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation [taken from NCBI Entrez Gene (Gene ID: 6923)].
UniGene ID
Hs.172772
UniProt ID (eCommerce)
Q15370

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.